目的 分离鉴定紫花高乌头总生物碱,研究其对大鼠慢性支气管炎的治疗作用机制。方法 采用薄层色谱法分离紫花高乌头总生物碱,利用各种波谱技术确定化合物的结构,并以脂多糖联合卡介苗诱导大鼠慢性支气管炎模型,地塞米松片及紫花高乌头总生物碱高和低剂量分别灌胃给药,HE法观察大鼠肺组织病理变化,按试剂盒要求用ELISA法测定血清中白细胞介素(IL)-1、IL-8、肿瘤坏死因子-α(TNF-α)水平变化。结果 从紫花高乌头总生物碱中分离鉴定了7个生物碱;紫花高乌头总生物碱能使大鼠支气管黏膜炎症细胞浸润程度减轻、支气管腔内分泌物减少、支气管黏膜上皮细胞脱落明显减轻,可抑制支气管黏膜上皮细胞增生,使支气管内径增大和通气量增大,而且紫花高乌头总生物碱可显著降低慢性支气管炎大鼠血清中升高的IL-1、IL-8和TNF-α 水平,与模型组比较有统计学意义(P＜0.05)。结论 紫花高乌头总生物碱对慢性支气管炎的治疗效果较好可能与其主要化学成分lappaconitine (1)、ranaconitine (2)、septentriodine (3)、6-demethyldelsodine (4)、6-methylumbrofine (5)、8-methyl-10-hydroxylycoctonine (6)和8-methyllycoctonine (7)相关。
Abstract
OBJECTIVE To isolate and identify the total alkaloids from Aconitum excelsum and to study the therapeutic mechanism of the total alkaloids from Aconitum excelsum on chronic bronchitis in rats. METHODS The total alkaloids from Aconitum excelsum was isolated by TLC and the structures of compounds were elucidated by a variety of spectral techniques. The rat model of chronic bronchitis (CB) was established with combined utilization of bacillus calmette-guerin and lipopolysaccharide, dexamethasone tablets and total alkaloids from Aconitum excelsum (high and low dose) were ig administrated. The pathological changes of lung tissue in rats were observed by HE and the level changes of interleukin-1 (IL-1), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in serum were determined by ELISA method. RESULTS Seven alkaloids were identified from the total alkaloids of Aconitum excelsum. The administration of total alkaloids from Aconitum excelsum could decrease the degree of infiltration, epithelial cell shedding, bronchial secretion, inhibit the proliferation of bronchial epithelial cells of the rat bronchial inflammatory cells, and make the bronchial diameter increases and ventilation. The total alkaloids could significantly inhibit the increase of IL-1, IL-8 and TNF-α in the serum of rats with CB, compared with the model group with the statistical significance (P<0.05). CONCLUSION The total alkaloids from Aconitum excelsum have the better treatment effect on CB, which may be related to its main chemical components, namely lappaconitine (1), ranaconitine (2), septentriod-ine (3), 6-demethyldelsodine (4), 6-methylumbrofine (5), 8-methyl-10-hydroxylycoctonine (6) and 8-methyllycoctonine (7).
关键词
蒙药紫花高乌头 /
总生物碱 /
慢性支气管炎 /
白细胞介素 /
肿瘤坏死因子-α
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Key words
Mongolian medicine Aconitum excelsum /
total alkaloids /
chronic bronchitis /
interleukin /
TNF-α
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中图分类号：
R965
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参考文献
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脚注
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基金
内蒙古民族大学科学研究项目资助(NMDYB1742)
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